PDCD1食蟹猴重組蛋白

蛋白名稱:PDCD1食蟹猴重組蛋白，90311-C08H
蛋白構(gòu)建:A DNA sequence encoding the cynomolgus PDCD1 (NP_001271065.1) (Met1-Gln167) was expressed with a polyhistidine tag at the C-terminus.
表達(dá)宿主:HEK293 Cells
蛋白純度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:

1. Immobilized PD-1 Protein, Cynomolgus, Recombinant (His Tag)(Cat:90311-C08H) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus PD-L1 / B7-H1 / CD274 Protein (Fc Tag)(Cat:90251-C02H),the EC50 is 16-86 ng/mL(QC tested). 

2. Immobilized PD-1 Protein, Cynomolgus, Recombinant (His Tag)(Cat:90311-C08H) at 2 μg/mL (100 μL/well) can bind Nivolumab-derived Anti-PD1, IgG4,the EC50 is 6-40 ng/mL(Routinely tested). 

3. Immobilized Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein (hFc & AVI Tag), Biotinylated, HPLC-verified (Cat: 90251-C41H-B) at 5 μg/mL (100 μl/well) on streptavidin precoated (5μg/mL, 100 μL/well) can bind Recombinant Cynomolgus PD-1 Protein (His Tag), HPLC-verified (Cat: 90311-C08H) , the EC50 is 187.75-563.55 ng/mL (Routinely tested).

4.Biotinylated Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein, hFc & AVI Tag (Cat. No. 90251-C41H-B) captured on Protein A chip, can bind Recombinant Cynomolgus PD-1 Protein, His Tag (Cat. No. 90311-C08H)  with an affinity constant of 1.200 μM as determined in an SPR assay (Biacore 8K) (Routinely tested).
蛋白內(nèi)毒素:< 1.0 EU per μg protein as determined by the LAL method.
預(yù)測(cè)N端:Leu 25
蛋白分子量:The recombinant cynomolgus PDCD1 consists of 154 amino acids and predicts a molecular mass of 17.4 kDa. As a result of glycosylation, it migrates as an approximately 36.7 kDa band in SDS-PAGE under reducing conditions.
蛋白NP號(hào):NP_001271065.1
蛋白氨基酸序列:Met1-Gln167
蛋白標(biāo)簽:C-His

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